The basic symbolic motifs in the seal are the cross, Scripture, the dove, and flames. Because of its association with Presbyterian history, the Celtic cross was chosen as a model for a more contemporary rendering of this ancient symbol. The two center lines of the cross represent an open book, emphasizing the central role of Scripture in the Reformed tradition.

The descending dove is the traditional symbol of the Holy Spirit. It also symbolizes Christ's baptism by John and the peace and wholeness which Christ's death and resurrection bring to a broken world. Beneath the image of the book is the suggestion of a lecturn or pulpit, which captures the important role of preaching in the history of Presbyterian worship.

Integrated into the lower part of the design are flames which form an implied triangle, a traditional symbol of the Trinity. The flames themselves convey a double meaning. They may be interpreted as a symbol of the revelation in the Old Testament when God spoke to Moses from the burning bush. The flames also suggest the beginning of the Christian church when Christ manifested himself to the apostles at Pentacost and charged them to be messengers of the good news of God's love.

The triangle also suggests the nature of Presbyterian government with its concern for balance and order, dividing power between ministers of the Word and lay people and between different governing bodies in the life of the church.

The seal of the Presbyterian Church (USA) was designed by Malcolm Grear Associates of Providence Rhode Island.